Journal: Materials Today Bio

Article Title: Ultrasound-activated piezoelectric Silk-PVDF hydrogel reprograms the osteoimmune microenvironment via NRF2 signaling for accelerated bone regeneration

doi: 10.1016/j.mtbio.2026.102779

Figure Lengend Snippet: Piezoelectric hydrogel activates NRF2 to attenuate ROS and macrophages polarization for osteogenesis . (A–B) RT-qPCR results for the mRNA expression of pro-inflammatory differentiation of macrophages. (C–D) RT-qPCR results for the mRNA expression of anti-inflammatory differentiation of macrophages. (E) The relative protein expression levels of INOS, CD206. (F–G) Semi-quantitative analysis of immunoblotting results of INOS, CD206. (H) ROS staining of ADSCs. (I) Mean intensity of ROS staining. (J) The relative protein expression levels of NRF2, NQO1, GPX4. (K–M) Semi-quantitative analysis of immunoblotting results of NRF2, NQO1, GPX4. Data are presented as the mean ± SEM; n = 3; ∗significant difference between selected groups, ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Membranes were blocked with 5 % non-fat milk (BD DifcoTM, #232100) in TBST (Beyotime, #ST673) for 1 h at 25 °C and incubated overnight at 4 °C with the following primary antibodies: Osteopontin (OPN) : • Rabbit monoclonal (Proteintech, 22952-1-AP), 1:1000 • Collagen I (COL1A1): Rabbit polyclonal (Proteintech, 14695-1-AP), 1:1000 • RUNX2: Rabbit monoclonal (Proteintech, 20700-1-AP), 1:1000 • NRF2: Rabbit monoclonal (Proteintech, 16396-1-AP), 1:2000 • NQO1: Mouse monoclonal (Proteintech, 67240-1-Ig), 1:2000 • Beta Actin: Mouse monoclonal (Proteintech, 66009-1-Ig), 1:20,000 After three 10-min TBST washes, membranes were incubated with HRP-conjugated goat anti-rabbit/mouse IgG (Beyotime, #A0208/#A0216, 1:5000) for 2 h at 25 °C.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining